How can integrated morphotaxonomy- and metabarcoding-based diatom assemblage analyses best contribute to the ecological assessment of streams?
نویسندگان
چکیده
Environmental conditions, such as nutrient concentrations, salinity, elevation etc., shape diatom assemblages of periphytic biofilms. These respond rapidly to environmental changes, a fact which makes diatoms valuable bioindicators. Hence, freshwater biomonitoring programmes currently use indices (e.g. EU Water Framework Directive - WFD). To date, microscopy-based assessments require high taxonomic expertise for identification at the species level. High-throughput technologies now provide cost-effective approaches that are promising, complementary or alternative tools bioassessment. The suitability metabarcoding method is evaluated first time in Cyprus streams WFD monitoring network, an eastern Mediterranean country with many endemic and results compared acquired from morphotaxonomic analysis. Morphotaxonomic was conducted microscopically, using most updated concepts, literature online resources. At same time, DNA involved rbc L 312 bp barcode, high-throughput sequencing bioinformatic ecological status calculated IPS Index. Results show positive correlation between morpho-taxonomic molecular scores. Discrepancies two methodologies related limitations both techniques. This study confirmed Fistulifera saprophila can have crucial role key differences observed, it negatively influences scores microscopy methods frequently overlook it. Importantly, gaps barcoding reference databases lead overestimation Overall, we conclude offsets methodology quality assessment freshwaters.
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ژورنال
عنوان ژورنال: Metabarcoding and Metagenomics
سال: 2021
ISSN: ['2534-9708']
DOI: https://doi.org/10.3897/mbmg.5.68438